Evaluation of Immunomodulatory activity of Diosgenin in rats
Main Article Content
Abstract
Background: The immune system is intrinsic to health. Modulation of the immune responses to alleviate the diseases by using herbal plants has been of interest for many years. Diosgenin, a naturally occurring steroid saponin mainly present in the seeds of fenugreek (Trigonella foenum graecum) and in the root tubers of wild yams (Dioscorea villosa). Activation of specific and nonspecific immunity results in stimulation of immune response. Diosgenin has the positive effects on both specific and nonspecific immunity. Aim: To study the immunomodulatory activity of Diosgenin in albino wistar rats. Method: The suspension of Diosgenin was given orally at the dosage level of 50, 100 and 150 mg/kg for 21 days in a rat. The immunomodulatory activity on specific and non-specific immunity was studied by heamagglutination antibody (HA) titer, delayed type hypersensitivity (DTH) response and carbon clearance test. Â Immunosuppression in a rat was induced by using Cyclophosphamide (100 mg/kg, p.o.). Sheep red blood cells (SRBCs) were used as antigen (0.1ml 20% SRBCs). Result: Diosgenin exhibited significant increase in the production of antibody titer in response to SRBC antigen. A significant increase in both primary and secondary HA titer was observed in immunosuppressed group treated with Diosgenin when compared with negative control. Â A significant increase in the DTH response was observed in immunosuppressed animals treated with Diosgenin, pre-sensitized with SRBCs antigen. Diosgenin exhibited significant increase in phagocytic index against control group, indicating the stimulation of the reticuloendothelial system. Conclusion: The study indicates that Diosgenin triggers stimulatory effect on specific and nonspecific immune response. The immunostimulant effect of Diosgenin could be attributed due to its saponin glycoside.
Article Details
How to Cite
Nimbalkar, V. V., Kadu, U. E., Shelke, R. P., Shendge, S. A., Tupe, P. N., & Gaikwad, P. M. (2018). Evaluation of Immunomodulatory activity of Diosgenin in rats. International Journal of Clinical and Biomedical Research, 4(3), 70-75. https://doi.org/10.31878/ijcbr.2018.43.15
Section
Original Research Articles
Copyright (c) 2018 Vikram V Nimbalkar, Urmila E Kadu, Ravina P Shelke, Suvarna A Shendge, Pratiksha N Tupe, Pandurang M Gaikwad
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2. Gokhale AB, Damre AS, Saraf MN. Investigations into the immunomodulatory activity of Argyreia speciosa. Journal of Ethnopharmacology. 2003;84:109-114.
3. Jawalkar PD, Bhujbal SS, Bafna AR. Immunomodulatory activity of herbal formulation (CE1 and CE2) containing Sphaeranthus indicus, Curculigo orchioides and Piper nigrum. International Journal of Pharmaceutical and Chemical Sciences. 2014;3(1):175-180.
4. Anarthe SJ, Malavika E, Pravalika A, Ganga MR. Screening of immunomodulatory activity of Sphaeranthus indicus Linn. whole plant. IOSR Journal of Pharmacy and Biological Sciences. 2015;10(6):91-99.
5. Dash S, Nath LK, Bhise S, Kar P, Bhattacharya S. Stimulation of immune function activity by the alcoholic root extract of Heracleum nepalense D Don. International Journal of Pharmacology. 2006;38(5):336-340.
6. Jashua Nfambl, Godfrey S. Bbosa, Lawrence Fred Sembalwe, James Gakunga, Josephine N. Kasolo. Immunomodulatory activity of methanolic leaf extract of Moringa oleifera in Wistar albino rats. Journal of Basic Clinical Physiology and Pharmacology. 2015;26(6):603–611.
7. Bilal Bin-Hafeez, Rizwanul Haque, Suhel Parvez, Suwarna Pandey, Iqbal Sayeed, Raisuddin S. Immunomodulatory effects of fenugreek (Trigonella foenum graecum L.) extract in mice. International Immunopharmacology. 2003;3:257–265.
8. Jayadev Raju, Mehta Rekha. Cancer chemopreventive and therapeutic effects of diosgenin, a food saponin. Nutrition and Cancer. 2009; 61(1): 27–35.
9. Yuan Q, Wu X, Huang W, Gong G, et al. Acute toxicity and sub-chronic toxicity of steroidal saponins from Dioscorea zingiberensis C.H. Wright in rodents. Journal of Ethnopharmacology. 2009;126(3):543–550.
10. Mallik A, Nayak S. Evaluation of immunomodulatory activity of Sesbania grandiflora flowers extract in mice. Indonesian Journal of Pharmacy. 2010;25(4):277–283.
11. Yadav R, Yadav N, Kharya MD. Immunomodulation potential of Andrographis Paniculata and Tinospora Cordifolia methanolic extracts in combination forms. International Journal of Pharmacological Research. 2016;6(1):29-40.
12. Dashputre NL, Naikwade NS. Immunomodulatory activity of Abutilon Indicum Linn on albino mice. International Journal of Pharmaceutical Sciences and Research. 2010; 1(3):178-184.
13. Kumar Pradeep Challa, Reddy Narsimha Yellu. In-vitro & in-vivo evaluation of immunomodulatory activity of flavanoidal constituents from berberis vulgaris root. International Journal of Pharmacy & Technology. 2013;5(3):5933-5943.
14. Nagarathna PKM, Reena K, Reddy Sriram, Wesley Johnson. Evaluation of immunomodulatory activity of the flavanoid of kigelia africana. International Journal of Pharmaceutical Sciences and Research. 2014;5(10):4359-4365.
15. Sudha P, Asdaq SB, Dhamingi SS, Chandrakala GK. Immunomodulatory activity of methanolic leaf extract of Moringa oleifera in animals. Indian Journal of Physiology and Pharmacology. 2010;54(2):133–140.
16. Rajan TV. The Gell-Coombs classification of hypersensitivity reactions:a re-intepretation. Trends in Immunology. 2003;24(7):376–379.
17. Dashputre NL, Naikwade NS. Preliminary immunomodulatory activity of aqueous and ethanolic leaves extracts of Ocimum basilicum Linn in mice. International Journal of PharmTech Research. 2010;2(2):1342-1349.